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. 2014 Feb 27;9(2):e90189. doi: 10.1371/journal.pone.0090189

Figure 8. Growth of extensions in PC12 cells after NGF stimulation.

Figure 8

PC12 cells seeded on different substrates were stimulated with NGF (50 ng/ml) in the absence of FBS and horse serum and images were captured every 2 days beginning at 3 days after NGF stimulation. Using AquaCosmos analysis software, the areas of differentiated cell bodies were compared to the areas of the outgrowth extensions. Representative data are shown in the graph. Cells cultured on an Si3N4 surface (○ ) or on a plastic culture dish (▵). The values shown are the mean ± S.E. of 30 samples. (*, p<0.001 vs. plastic culture dish).