Figure 4. Comparison of the segmentation quality achieved by the investigated algorithms on 2D benchmark images from the Broad Bioimage Benchmark Collection (BBBC006v1).

Original image (A), adaptive thresholding using Otsu’s method [23] (B), Otsu’s method combined with watershed-based blob splitting [23], [30] (C), geodesic active contours [31] (D), gradient vector flow tracking [16] (E), graph-cuts segmentation [17] (F), TWANG segmentation (G) and a false colored original image (H). The symbols indicate segmentation errors for nuclei that are either split (#), merged (+), missing (o) or spurious (∼).