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. 2014 Feb 22;14:119. doi: 10.1186/1471-2407-14-119

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Copyright © 2014 Lehn et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ER and PgR protein levels increase upon YAP1 downregulation. (a) Immunocytochemical stainings of the T47D cell line. Cells were transfected with the indicated siRNA and 48 hours later, medium was changed to phenol red-free DMEM supplemented with 5% charcoal stripped serum in which cells were grown for 24 hours. Subsequently, 10^-9 M E2 or 10^-9 M E2 combined with 10^-7 M 4-OH-tam was added and 4 days later cells were harvested, fixed and stained using the indicated antibody. (b) Quantification by automated image analysis of stained cell pellets. Bars represent the percentage of positive pixels identified in the image analysis. ER = estrogen receptor α, PgR = progesterone receptor, 4-OH-tam = 4-hydroxi-tamoxifen, E2 = 17β-estradiol.