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. Author manuscript; available in PMC: 2015 Jan 21.
Published in final edited form as: Biochemistry. 2014 Jan 8;53(2):361–375. doi: 10.1021/bi401404u

Figure 6. Heparin binding ability of unmodified and acrolein-modified apoE.

Figure 6

Unmodified (a) or acrolein-modified (b) apoE (100 μg) was loaded onto a heparin-Sepharose column in 20 mM sodium phosphate pH 7.4 in an ÄKTA FPLC system. The flow rate was maintained at 1 ml/min. A salt gradient of 0 – 1.0 M NaCl was used to elute the bound protein; the elution of the protein was monitored at 280 nm. The arrow represents the start of the gradient.