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Electrophoresis gel plot of single-gene and multiplex PCR using C. difficile reference strain 630 and the NAP1/027/BI cultured isolate VA17. The template is indicated at the top of each lane. Lanes 1 and 4, tcdB primer set; lanes 2 and 5, tcdC primer set; lanes 3 and 6, cdtB primer set; lanes 7 and 8, multiplex results for the reference strain 630 and the hypervirulent VA17 isolate, respectively.
