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. 2014 Mar;16(2):244–252. doi: 10.1016/j.jmoldx.2013.11.006

Figure 3.

Figure 3

A: Electrophoresis gel plot of multiplex PCR. A different clinical stool DNA isolate was used as template for each amplification, from eight positive samples and two negative controls. A sample identifier for the template is indicated at the top of each lane. Each positive sample (lanes 1 to 8; samples A–C, E–G, L, and M) shows the 328-bp amplicon for tcdB, followed by either a 300-bp amplicon for tcdC (lanes 1, 3, 7, and 8; samples A, C, L, and M) or a 282-bp amplicon for tcdC with the NAP1/027/BI deletion (lanes 2, 5, and 6; samples B, F, and G). Sample E (lane 4) shows a greater deletion in tcdC at 261 bp. Samples with any deletion in tcdC were also positive for binary toxin, indicated by the amplicon at 190 bp for cdtB (B, E, F, and G). Lanes 9 and 10 (samples N and O) show representative samples of clinical negative controls. B: Electropherogram for samples E, F, and L. The electropherogram image shows the peaks of the three amplicons cdtB, tcdC, and tcdB for samples E, F, and L, respectively. The base-pair shifts in the tcdC amplicon are easily visible between 70.0 seconds and 77.5 seconds; sample L shows the longest amplicon at 75.25 seconds, followed by sample F (18-bp deletion) at 73.55 seconds and then sample E (39-bp deletion) at 71.25 seconds. The peak for tcdB appears in all three samples at 79.0 seconds and cdtB in samples E and F at 64.4 seconds.