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. 2014 Mar;16(2):244–252. doi: 10.1016/j.jmoldx.2013.11.006

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© 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

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A: Singleplex qPCR results for tcdB, tcdC, and cdtB amplification in the VA17 NAP1/027/BI hypervirulent strain of C. difficile. The tcdB, tcdC, and cdtB amplicons have C_T values of 11.8, 15.2, and 11.3, respectively, for a starting concentration of 1.0 ng/μL. B: The corresponding electrophoresis gel plot for PCR performed on the droplet sandwich platform shows, the amplicons are of the appropriate size: 328 bp for tcdB, 282 bp for tcdC, and 190 bp for cdtB. The amplicon size of tcdC indicates the presence of the 18-bp deletion associated with NAP1/027/BI.