Table 1.

LR-PCR Primers for NGS Sequencing

Fragment	Primers	Sequence	Size (kb)	Genomic location
PKD1_Ex1	PKD1_NGS_1F	5′-CGCAGCCTTACCATCCACCT-3′	2.3	chr16:2185030-2187307
	PKD1_NGS_1R	5′-TCATCGCCCCTTCCTAAGCA-3′
PKD1_Ex2-12	PKD1_NGS_2-12F	5′-CCAGCTCTCTGTCTACTCACCTCCGCATC-3′	8.7	chr16:2163080-2171636
	PKD1_NGS_2-12R	5′-CCACGGTTACGTTGTAGTTCACGGTGACG-3′
PKD1_Ex13-21	PKD1_NGS_13-21F	5′-TGGAGGGAGGGACGCCAATC-3′	7.9	chr16:2155145-2163036
	PKD1_NGS_13-21R	5′-ACACAGGACAGAACGGCTGAGGCTA-3′
PKD1_Ex22-34	PKD1_NGS_22-34F	5′-ATGCTTAGTGAGGAGGCTGTGGGGGTC-3′	7.8	chr16:2146980-2154794
	PKD1_NGS_22-34R	5′-ATGAGGCTCTTTCCACAGACAACAGAGGTT-3′
PKD1_Ex35-46	PKD1_NGS_35-46F	5′-CTGTGGGCGATGGGTTTATCAGCAG-3′	5.2	chr16:2139301-2144473
	PKD1_NGS_35-46R	5′-GAGACGGTGCAGGGAGTACGGTAGGA-3′
PKD2_Ex1	PKD2_NGS_ExP-1_F	5′-GTGGAGACAGAAGCCAACCAAAGAG-3′	1.4	chr4:88928226-88929584
	PKD2_NGS_ExP-1_R	5′-GGATGCGAGATGGAGCCCG-3′
PKD2_Ex2	PKD2_NGS_Exon2_F	5′-TTTCTTTCCATTTGCAATGTTTCATTC-3′	2.5	chr4:88938491-88940897
	PKD2_NGS_Exon2_R	5′-GGAAGATAGTCAATAAACAAATGCCCAA-3′
PKD2_Ex3-6	PKD2_NGS_Ex3-6_F	5′-GAGAAGACCTTGTGTGAATTTGTCCA-3′	10.9	chr4:88957246-88968207
	PKD2_NGS_Ex3-6_R	5′-TCATACTCAGCAAAGTTACTCATGCAAA-3′
PKD2_Ex7-10	PKD2_NGS_Ex7-10_F	5′-TCGGGTAAGTATAATGGTGAGCCCT-3′	10.3	chr4:88973018-88983323
	PKD2_NGS_Ex7-10_R	5′-CATCAAGACTCCAAGATAGGGAACATTT-3′
PKD2_Ex11-15	PKD2_NGS_Ex11-15_F	5′-CACGTACTTGTTGAATGGCCAATGT-3′	10.8	chr4:88986401-88997197
	PKD2_NGS_Ex11-15_R	5′-ATGAAACTCAGAAGCCCTTTGACAGTT-3′