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NGS Analytic Sensitivity and Specificity (Variants Detection)
| NGS | Sanger sequencing |
||
|---|---|---|---|
| Variant alleles (positive) | Reference alleles (negative) | Total | |
| Variant alleles (positive) | 248 | 0 | 248 |
| Reference alleles (negative) | 2 | 1825 | 1827 |
| Total | 250 | 1825 | 2075 |
Compared with the Sanger sequencing assay: sensitivity = 99.2% (95% CI, 96.8%–99.9%); specificity = 99.9% (95% CI, 99.7%–100.0%).
