Table 4.
Cobalamin biosynthesis genes which change in regulation following exposure of strain 6179 to BZT.
| Functional category | Locus tag | Gene | Protein | Fold change |
|---|---|---|---|---|
| UPREGULATED | ||||
| Cobalamin biosynthesis | LM6179_1509 | cbiK | Sirohydrochlorin cobaltochelatase | 15.24 |
| LM6179_1510 | cbiL | Precorrin-2 C20-methyltransferase | 12.46 | |
| LM6179_1506 | cbiH | Cobalt-precorrin-3B C(17)-methyltransferase | 8.73 | |
| LM6179_1504 | cbiF | Cobalt-precorrin-4 C(11)-methyltransferase | 5.97 | |
| LM6179_1505 | cbiG | CbiG protein | 7.05 | |
| LM6179_1501 | cbiD | Putative cobalt-precorrin-6A synthase (deacetylating) | 8.40 | |
| LM6179_1507 | cbiJ/cobK | Precorrin-6X reductase | 9.35 | |
| LM6179_1502 | cbiE | Putative cobalt-precorrin-6Y C(5)-methyltransferase | 10.05 | |
| LM6179_1503 | cbiT | Putative cobalt-precorrin-6Y C(15)-methyltransferase (decarboxylating) | 10.27 | |
| LM6179_1500 | cbiC | Cobalt-precorrin-8X methylmutase | 8.66 | |
| LM6179_1498 | cobB/cbiA | Cobyrinic acid A,C-diamide synthase | 6.61 | |
| LM6179_1508 | cobA | Uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase | 11.09 | |
| LM6179_1499 | cobD | Cobalamin biosynthesis protein CobD | 9.28 | |
| LM6179_1515 | cobQ | Cobyric acid synthase | 6.25 | |
A gene is considered to be up-regulated if its expression level is greater when the bacterium is grown in the presence of BZT than in the absence of BZT.