Table 7.
Stress response, multi-drug resistance transporter, and phage/prophage genes which change in regulation following exposure of strain 6179 to BZT.
| Functional category | Locus tag | Gene | Protein | Fold change |
|---|---|---|---|---|
| UPREGULATED | ||||
| Stress response | LM6179_2107 | cspC | Cold-shock protein | 9.25 |
| LM6179_2786 | cspD | Cold-shock protein, molecular chaperone, RNA-helicase co-factor | 4.84 | |
| LM6179_1203 | rsbRA | Component of the piezosome (stressosome), positive regulation of sigma(B) activity in response to salt and heat stress | 4.22 | |
| LM6179_0510 | ftsH | Cell-division protein and general stress protein (class III heat-shock) | 3.70 | |
| LM6179_0506 | hslR | Ribosomal RNA binding protein involved in 50S recycling, heat shock protein | 3.53 | |
| LM6179_0512 | hslO | Disulfide bond chaperone (heat shock protein HSP33) | 3.40 | |
| LM6179_2585 | yloU | Putative alkaline-shock protein | 4.76 | |
| Multi-drug resistance | LM6179_2296 | qacH | Quaternary ammonium compound-resistance protein | 4.01 |
| Transporters | LM6179_1165 | ykkC | Efflux transporter | 3.71 |
| LM6179_0824 | lmrB | Efflux transporter, drug-export protein | 3.25 | |
| LM6179_1166 | ykkD | Efflux transporter | 2.75 | |
| LM6179_2153 | LM6179_2153 | Multidrug resistance transporter | 2.23 | |
| LM6179_1610 | ycnB | Putative efflux transporter | 2.08 | |
| LM6179_1298 | LM6179_1298 | Drug resistance transporter, EmrB/QacA family | 1.76 | |
| LM6179_1894 | yuxJ | Putative exporter | 1.69 | |
| LM6179_0246 | LM6179_0246 | Efflux protein | 1.50 | |
| LM6179_0155 | mdtG | Putative metabolite efflux transporter | 1.27 | |
| LM6179_2368 | ycnB | Uncharacterized MFS-type transporter ycnB | 1.20 | |
| DOWNREGULATED | ||||
| Phage/prophage | LM6179_1786 | LM6179_1786 | Bacteriophage major tail shaft protein | −11.35 |
| LM6179_1780 | LM6179_1780 | Phage—major head protein | −7.45 | |
| LM6179_1597 | LM6179_1597 | Phage tail tape measure protein | −6.60 | |
| LM6179_1588 | LM6179_1588 | Cps major phage capsid protein | −4.98 | |
| LM6179_1968 | LM6179_1968 | Phage holin protein | −14.49 | |
| LM6179_1777 | LM6179_1777 | Phage portal protein | −5.66 | |
| LM6179_1580 | LM6179_1580 | Phage-related transcriptional activator | −3.74 | |
A gene is considered to be up-regulated if its expression level is greater when the bacterium is grown in the presence of BZT than in the absence of BZT.