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Experiment using real Physarum (scale bar: 10 mm). The radius of a pac-man was 5 mm, and the distance between the centers of two pac-men was 15 mm. (A) The captured image. (B) Obtained by subtracting the background of (A). (C) Adjusted the brightness. (D) Binarization. All processes were executed by using the ImageJ software. In the binary image, the plasmodia are indicated by black pixels. The threshold of binarization is determined not to delete delicate structure of plasmodium.
