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. 2008 Jul 2;80(2):280–289. doi: 10.1093/cvr/cvn182

Figure 1.

Figure 1

Effects of aging in aldehyde dehydrogenase (ALDH-2)- or manganese superoxide dismutase (MnSOD) deficient mice on mitochondrial oxidative stress. Reactive oxygen species (ROS) formation in cardiac mitochondria (0.1 mg/mL final protein) was measured by L-012 (100 µM) enhanced chemiluminescence in the presence of succinate (5 mM). Mitochondria from young, middle-aged, and old B6WT mice (A), ALDH-2−/− mice (B6WT background) (B) and middle-aged vs. old MnSOD+/+/MnSOD+/− mice (B6xOla background) (C) were compared with respect to ROS formation. Data are means ± SEM of indicated number of independent experiments performed with mitochondria from two to three pooled hearts from animals from the same group on the same day. Different strains were measured at different time points. *P < 0.05 vs. young group or MnSOD+/+ mice and #P < 0.05 vs. middle-aged group.