FIGURE 7.
γ1 subunits slow synaptic current kinetics. A, schematic representation of the subunit chimeras used to investigate γ subunit related synaptic clustering. Swapping the intracellular and TM4 domains made the chimeras. B, averaged synaptic currents recorded from heterosynapses expressing the wild-type α2β2γ2L and α2β2γ1 (reproduced from Fig. 5) and chimeric α2β2γ1-γ2L and α2β2γ1-γ2L GABAARs. Note the similar activation and deactivation between wild-type and corresponding chimeric GABAARs. C, averaged values for activation and deactivation for the wild-type and chimeric GABAARs showing that both activation and deactivation are strongly dependent on the ID plus TM4 domains of the γ subunit.