Skip to main content
. 2014 Jan 8;289(9):5499–5509. doi: 10.1074/jbc.M113.532143

FIGURE 2.

FIGURE 2.

Serglycin is secreted by myeloma cells and is present on the cell surface. A, Western blot analysis for the presence of serglycin in the conditioned medium from five different human myeloma cell lines. Culture medium without cells is used as the control. B, serglycin expressed by myeloma cell lines has side-chains of CS glycosaminoglycan. Conditioned medium of KMS11 human myeloma cell line was either treated or not treated with the CS-degrading enzyme chondroitinase ABC (Chase ABC) and then analyzed for serglycin by Western blotting. C, flow cytometric analysis demonstrating serglycin expression on the surface of myeloma cell lines (CAG, KMS11, RPMI8226, OPM1, and OCImy5). Solid curves represent isotype control, and open curves indicate serglycin staining. D, serglycin binding to myeloma cell surface is dependent on their CS chains. OCImy5 human myeloma cell line was either treated or not treated with Chase ABC enzyme and then analyzed for cell surface serglycin by flow cytometry. Black curves represent isotype control, gray curves indicate serglycin level after Chase ABC digestion, and open curves represent serglycin level without ABC digestion.