FIGURE 4.

Knockdown of serglycin expression diminishes subcutaneous growth of myeloma cells. A, knockdown of serglycin expression does not alter the in vitro growth of myeloma cells. Control or serglycin-knockdown CAG cells were seeded at equal density, and the growth in cell numbers was assessed by MTT assay at 0, 1, 2, and 3 days. Points represents mean of triplicate samples ±S.D. from each of two separate experiments. B, 6 weeks after subcutaneous injection of control and serglycin-knockdown CAG cells into flanks of male SCID mice, the tumors were removed at necropsy and photographed (upper panel). “X” represents animals with no detectable tumor. Lower panel represents the immunohistochemistry of sections from xenograft tumors formed from control or serglycin-knockdown cells stained for human serglycin (brown) and counterstained with hematoxylin (blue). Scale bars, 200 μm. C, levels of human kappa light chain in the serum were measured by ELISA. Data include all animals that were injected with tumor cells and represents mean ± S.D. of duplicate from a single ELISA.