FIGURE 1.

Impact of SREBP-1 knock-down on carbohydrate metabolism in normal mice. Groups of five C57BKS mice were given 1 × 10¹¹ viral particles of gAd.shSCR or gAd.shSREBP1 and euthanized 1 week later under fed or 24-h fasted conditions. A, helper-dependent adenovirus vector used to express shRNA. Hairpin RNA expression was driven by the U6 promoter. ITR, inverted terminal repeat; Ψ, packaging signal. B, SREBP-1 precursor (p-SREBP) or nuclear (n-SREBP) protein levels. C, blood glucose unaffected by SREBP-1 silencing. Glycogen levels were significantly lower under fed and fasted conditions in mice treated with gAd.shSREBP1; *, p < 0.05; n = 5–6; error bars, S.D. D, immunoblots to detect GYS2, P-GYS2, PCK1, and tubulin.