FIGURE 2.

SREBP1 silencing in db/db mice. Groups of eight db/db mice were given 1 × 10¹¹ viral particles of gAd.shSCR or gAd.shSREBP1 through the tail vein, and euthanized 3 weeks later under fed (ad libitum) or 24-h fasted conditions. Groups of four normal C57BLKS mice were used as controls and received the same dose of gAd.shSCR adenovirus. A, effect of silencing SREBP1 on lipogenic enzyme expression. Data are presented as mean ± S.D. (error bars). *, p < 0.05 versus control; n = 7–8. AU, arbitrary units. B, immunoblot analysis for SREBP-1 precursor (p-SREBP) or nuclear (n-SREBP) protein, ACAC, and phosphorylated ACAC (P-ACAC), 3 weeks after gAd.shSCR or gAd.shSREBP1 administration. ACAC and P-ACAC panels show ACACA as a bottom 265-kDa band and ACACB as a top 280-kDa band. C, hepatic triglyceride levels in db/db mice; *, p < 0.05 versus control; n = 8.