FIGURE 3.

SREBP-1 silencing decreases de novo lipogenesis. A, groups of 8 db/db mice were administered 1 × 10¹¹ viral particles of gAd.shSCR or gAd.shSREBP1. Three weeks later, ²H₂O was administered intraperitoneally. Mice were fasted for 4 h and euthanized. Total palmitate, de novo synthesis of palmitate, malonyl-CoA, and BHB-CoA were analyzed (p < 0.05 versus control; n = 8). B, mice were treated as described in Fig. 2. CPT1A and CPT2 protein levels under fed and fasted conditions and CPT1A sensitivity to malonyl-CoA in livers of mice fasted for 24 h were determined. n = 5. C, mice were treated as described in Fig. 2. BHB serum levels are shown; n = 4–5 WT, n = 8 db/db. D, LDL receptor (Ldlr) mRNA expression in db/db mice; n = 7–8. AU, arbitrary units.