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. 2014 Jan 16;289(9):5537–5548. doi: 10.1074/jbc.M113.541466

FIGURE 2.

FIGURE 2.

SDS-PAGE analysis of RFC complexes. SDS-PAGE of the final purified protein complexes stained with Coomassie Brilliant Blue is shown for WT RFC and each of the mutants. Subunit molecular masses are as follows: Δ283 Rfc1, 65 kDa; Rfc2, 40 kDa; Rfc3, 38 kDa; Rfc4, 36 kDa; and Rfc5, 40 kDa. Because of their similarity in size, the bands corresponding to the Rfc2, Rfc3, and Rfc5 subunits overlap.