TABLE 3.

Anisotropy-based equilibrium DNA binding

Reactions contained 25 nm DNA-RhX, 2 μm WT PCNA, 0–2 μm clamp loader, and 0.5 mm ATPγS.

Clamp loader	RFC only Kd	RFC + PCNA Kd
	nm	nm
WT	120 ± 20	60 ± 20
Rfc1GAT	230 ± 20	440 ± 40
Rfc2GAT	140 ± 50	340 ± 120
Rfc3GAT	130 ± 50	300 ± 170
Rfc4GAT	230 ± 30	570 ± 150
WT no ATPγS	800 ± 150	4700 ± 4900