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. 2014 Jan 15;289(9):5609–5618. doi: 10.1074/jbc.M113.509380

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Rpn13 co-immunoprecipitates with hybrid proteasome containing the PA28 complex. 293 cells were transiently transfected with the indicated expression plasmids. Total cellular content was revealed directly (lanes 4–6; input) or were subjected to a FLAG affinity purification (lanes 1–3). PA28α-FLAG served as a positive control for the ability to purify hybrid proteasomes as indicated by the presence of 20S (PSMA1) and 19S (PSMD14) subunits. PA28β-HA served as a negative control for the FLAG immunoprecipitation when expressed without PA28α. An asterisk indicates a nonspecific band.