FIGURE 3.

Far-UV CD of PFL-AE in the absence (dotted line) and presence of AdoMet (solid line) under anaerobic conditions in 20 mm HEPES, 250 mm NaCl, pH 7. 4 at 25 °C. CD parameters were as follows: sensitivity was 100 millidegrees, data pitch was 0.1 nm, scan range was 195–260 nm, in continuous scan mode at a rate of 50 nm/minute, response of 4 s, bandwidth of 0.1 nm, and an accumulation of three scans; all measurements were performed using a 0.1-mm path length cuvette. M.R.E., mean residue ellipticity.