FIGURE 8.

The LPA-Cyr61 pathway mediated LPA-induced FAK activation and cell migration via integrins α6β1 and ανβ3. A, The specific antibodies against integrin (Itg) α6, β1, or ανβ3, but not the β4 and β5 antibodies, blocked LPA-induced SMC migration. IgG was used as a control. B and C, knockdown of integrins (Itg) α6, β1, and β3 with specific siRNAs blocked LPA-induced SMC migration. Knockdown efficiency of integrin expression was assessed with Western analysis (B). Cell migration analysis was performed using a Transwell chamber assay. D, Western blot results indicate that knockdown of integrins α6, β1, or β3 blocked LPA-induced FAK activation. P, phosphorylated.