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. 2014 Jan 14;289(9):5784–5798. doi: 10.1074/jbc.M113.483115

FIGURE 8.

FIGURE 8.

Knockdown of Mstn by shRNA partially attenuated CMM-induced DNA damage. A, representative Western blot (i) and densitometric analysis (ii) showing p63, REDD1, p-Histone H2A.X and H2A.X protein levels in 48 h proliferating C2C12 myoblasts transfected with empty vector control (pGFP-V-RS) (lanes 1–3), scrambled shRNA (lanes 4–6), or shMstn vector (lanes 7–9) and treated with two different concentrations of CMM; lanes 1, 4, 7-CCM-treated, lanes 2, 5, 8-CMM1-treated, lanes 3, 6, 9-CMM2-treated. GAPDH was used as internal loading control on the gel (*, p < 0.05, **, p < 0.01 when compared with empty vector control transfected-CCM-treated cells; ^, p < 0.05 when compared with shMstn-transfected-CCM-treated cells) (n = 3).