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. 2013 Dec 23;289(9):5828–5845. doi: 10.1074/jbc.M113.478495

FIGURE 2.

FIGURE 2.

Effect of ORG27569 on CP55,940-induced [35S]GTPγS binding, as well as the effect on basal levels of [35S]GTPγS binding, in wild-type and hCB1R cells expressing the K3.28192A mutant. A, CP55,940-induced [35S]GTPγS binding when applied alone (DMSO) or in the presence of ORG275659 (1 μm). The effect of CP55940 is expressed as a percentage of the Emax value of CP55940 in the presence of vehicle. B, effect of ORG27569 and SR141617A on basal levels of [35S]GTPγS binding in wild-type (WT) cells. C, CP55,940-induced [35S]GTPγS binding when applied alone (DMSO) or in the presence of ORG275659 (1 μm) in K3.28192A mutant hCB1R cells. The effect of CP55940 is expressed as a percentage of the Emax value of CP55940 in the presence of vehicle. D, effect of ORG27569 on basal levels of [35S]GTPγS binding in WT and K3.28192A mutant hCB1R cells is illustrated here. Data were analyzed via one sample t test compared with zero where **, p ≤0.01; ***, p ≤0.001.