TABLE 2.
Signal transduction of hY4 receptor mutants, mutated residues located on TM2
The immunoprecipitation accumulation assays were performed using increasing concentrations of hPP, [Ala27]hPP, [Leu27]hPP, [Cha27]hPP, and [Phe27]hPP. The incubation time lasted for 1 h. EC50 values from dose-response curves were determined. n represents the number of independent experiments, each performed in duplicate.
| Peptides | Wild type |
Y2.64A |
Y2.64L |
Y2.64F |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EC50 (nm),a (pEC50 ± S.E.) | EC50 ratio,b (mutant/wild type) | n | EC50 (nm),a (pEC50 ± S.E.) | EC50 ratio,b (mutant/wild type) | n | EC50 (nm),a (pEC50 ± S.E.) | EC50 ratio,b (mutant/wild type) | n | EC50 (nm),a (pEC50 ± S.E.) | EC50 ratio,b (mutant/wild type) | n | |
| hPP | 1.44 (8.84 ± 0.04) | 1 | 39 | 93.20 (7.03 ± 0.09) | 65 | 7 | 1.30 (8.89 ± 0.11) | 0.9 | 4 | 6.13 (8.21 ± 0.09) | 4 | 5 |
| [Ala27]hPP | 11.78 (7.93 ± 0.08) | 8 | 4 | 579.90 (6.24 ± 0.15) | 424 | 4 | 205.20 (6.69 ± 0.09) | 138 | 3 | NTc | ||
| [Leu27]hPP | 1.19 (8.92 ± 0.17) | 0.85 | 4 | 90.63 (7.04 ± 0.14) | 63 | 4 | 32.20 (7.49 ± 0.09) | 21 | 3 | NT | ||
| [Cha27]hPP | 2.81 (8.55 ± 0.09) | 2 | 7 | 22.34 (7.65 ± 0.17) | 14 | 4 | 33.45 (7.48 ± 0.14) | 23 | 2 | NT | ||
| [Phe27]hPP | 0.852 (9.07 ± 0.12) | 0.6 | 4 | NT | NT | 11.26 (7.95 ± 0.11) | 9 | 4 | ||||
a EC50 and pEC50 values were determined from the mean ± S.E. of n independent experiments, each performed in duplicate.
b The ratio was calculated using the global fitting function for EC50 shift determination of Prism 5.03.
c NT represents not tested.