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. 2014 Jan 5;289(9):5960–5969. doi: 10.1074/jbc.M113.532234

FIGURE 7.

FIGURE 7.

Identification of STAT1 and NF-κB binding sites in human lipocalin-2 promoter. A, human subcutaneous adipocytes were untreated or treated with 100 ng/ml recombinant human IFNγ for 30 min and then cross-linked with formaldehyde. Chromatin extracts were prepared and immunoprecipitated with STAT1 antibody. IP products were subjected to quantitative real time PCR analysis. The percentage of input was calculated and normalized to the control group. B, human subcutaneous adipocytes were untreated or treated with 0.5 nm recombinant human TNFα for 30 min and cross-linked with formaldehyde. Chromatin extracts were isolated and subjected to immunoprecipitation with NF-κB p65 antibody. IP products were analyzed by real time PCR. The percentage of input was calculated and normalized to the control. ***, p < 0.001. Error bars represent S.D. CTL, control.