FIGURE 10.
PIP2 depletion from PM disables exogenous Syn-1A from further inhibiting KATP channels. A, representative KATP channel current tracings of control, GST-Syn-1A (1 μm infused into cells via patch pipette), synaptojanin-1, and synaptojanin + GST-Syn-1A in INS-1 cells. B, summary results showing that exogenous GST-Syn-1A could not cause further inhibition of KATP channels when endogenous PIP2 levels were depleted by synaptojain-1. Results are mean ± S.E.; *, p < 0.05; **, p < 0.01. NS, not significant.