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. 2014 Jan 15;289(9):6028–6040. doi: 10.1074/jbc.M113.511808

FIGURE 6.

FIGURE 6.

Syn-1A-5RK/A inhibition of KATP channels is resistant to physiologic low PIP2 (1 μm) but not high PIP2 (10 μm) opening of KATP channels. A, representative KATP channel current tracings of Syn-1A-WT- (left traces) and PIP2-insensitive Syn-1A-5RK/A-expressing INS-1 cells (right traces), treated with or without 1 μm PIP2 (in pipette solution). Note PIP2 partial reversal of Syn-1A inhibition (left traces) but not of Syn-1A-RK/A inhibition (right traces). B, representative KATP channel current traces from control (left), Syn-1A WT (middle), and Syn-1A-5RK/A (right)-overexpressing INS-1 cells treated without or with 10 μm PIP2 (in pipette solution). C, summary of A, n = 6–12 cells. D, summary of B showing no difference in current densities between control (n = 8), Syn-1A WT-expressing (n = 9), and Syn-1A-5RK/A-expressing (n = 11) cells. Results are mean ± S.E. (error bars); *, p < 0.05; NS, not significant.