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. 2014 Jan 17;289(9):6152–6164. doi: 10.1074/jbc.M113.533562

FIGURE 3.

FIGURE 3.

Recarboxylation of the lysine can restore the enzyme activity in OXA-24 but not in OXA-1. A, black line, 1 μm OXA-24 was incubated with 100 μm NCF in 10 mm HEPES buffer (pH 7.5); red line, 1 μm OXA-24 was first incubated with 2 mm penem 1, and then 100 μm NCF was added; blue line, reagents were added in the order of 1 μm OXA-24, 2 mm penem 1, 100 μm NCF, and 100 mm NaHCO3. B, black line, 1 μm OXA-1 was incubated with 100 μm NCF in 10 mm HEPES buffer (pH 7.5); red line, 1 μm OXA-1 was first incubated with 4 μm penem 1, and then 100 μm NCF was added; blue line, reagents were added in the order of 1 μm OXA-1, 4 μm penem 1, 100 μm NCF, and 100 mm NaHCO3.