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. 2013 Dec 12;7(2):177–183. doi: 10.1111/1751-7915.12098

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© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Evaluation of engineered green-light sensing system in Synechocystis sp. PCC6803 under red or green/red light.A. Schematic representation of each construct introduced into pKT230: P_cpcG₂-GFPuv (PcpcG2), P_cpcG₂-GFPuv-P_ccaS-ccaS (PcpcG2+ccaS), P_cpcG₂-GFPuv-P_ccaR-ccaR (PcpcG2+ccaR).B. Relative normalized fluorescence intensity of Synechocystis sp. PCC6803 harbouring PcpcG2, PcpcG2+ccaS, or PcpcG2+ccaR under red or green/red light. Each value is normalized to cell optical density and provided relative to the normalized value of PcpcG2 under green light (set as 1).C. Relative normalized fluorescence intensity of Synechocystis sp. PCC6803 harbouring PcpcG2, pKT230-P_cpcG₂-SD-GFPuv (PcpcG2-SD), P_cpcG₂-SD-GFPuv+ccaR (PcpcG2-SD+ccaR) under red or green/red light. Each value is normalized to cell optical density and provided relative to the normalized value of PcpcG2 under green light (set as 1).D. Relative normalized fluorescence intensity of Synechocystis sp. PCC6803 harbouring pKT230-P_trc-SD-GFPuv (Ptrc-SD), whose SD-like sequence was replaced by the SD-like sequence of cpcB, and P_cpcG₂-SD+ccaR. Each value is normalized to cell optical density.