Table 1.
Strains and plasmids used in this study.
| Name | Description | Source |
|---|---|---|
| Strains | ||
| MG1655 | E. coli MG1655 wild type | Laboratory stock |
| ΔarcZ | MG1655 ΔarcZ::kanR (promoter-10 mutant) | This study |
| ΔdsrA | MG1655 ΔdsrA::kanR | This study |
| ΔrprA | MG1655 ΔrprA::kanR | This study |
| MG1655Δ3 | MG1655 ΔdsrA ΔrprA ΔarcZ::kanR | This study |
| BW25113ΔrpoS | E. coli BW25113 ΔrpoS::kanR | Keio collection (Baba et al. 2006) |
| BW25113ΔarcB | E. coli BW25113 ΔarcB::kanR | Keio collection (Baba et al. 2006) |
| BW25113ΔgadX | E. coli BW25113 ΔgadX::kanR | Keio collection (Baba et al. 2006) |
| BW25113ΔgadW | E. coli BW25113 ΔgadW::kanR | Keio collection (Baba et al. 2006) |
| BW25113Δhfq | E. coli BW25113 Δhfq::kanR | Keio collection (Baba et al. 2006) |
| ΔrpoS | E. coli MG1655 ΔrpoS::kanR | This study |
| ΔarcB | E. coli MG1655 ΔarcB::kanR | This study |
| ΔgadX | E. coli MG1655 ΔgadX::kanR | This study |
| ΔgadW | E. coli MG1655 ΔgadW::kanR | This study |
| Δhfq | E. coli MG1655 Δhfq::kanR | This study |
| SG30013 | MG1655 lacX74 attRpoS750::LacZ, Reporter for transcription, translation, and degradation of RpoS | S. Gottesman (Wassarman et al. 2001) |
| Plasmids | ||
| pHMB1 | A derivative of pHM1 (Han et al. 2010), AmpR, IPTG-inducible transcription from immediate after EcoRI site, modified rnpB terminator (GAUUU to GGAGU) next to XbaI site. | This study |
| pArcZ | pHMB1 carrying arcZ | This study |
| pDsrA | pHMB1 carrying dsrA | This study |
| pRprA | pHMB1 carrying rprA | This study |
| pGEM3 | Cloning vector, AmpR | Promega |
| pGEM-T | Cloning vector, AmpR, Site-directed mutagenesis for arcZ | Promega |
| pG-ArcZ | pGEM3 carrying arcZ | This study |
| pBAD-myc-hisC | Control vector for pBAD-RpoS plasmid | Invitrogen |
| pBAD-RpoS | rpoS cloned in pBAD24, AmpR | Zhou and Gottesman (2006) |
| pKD46 | λRed recombination system under control of arabinose-inducible promoter, temperature-sensitive replication, AmpR | Datsenko and Wanner (2000) |
| pKD13 | Template plasmid for FRT-flanked kanamycin resistance cassette. | Datsenko and Wanner (2000) |
| pCP20 | FLP+, λ cI857+, λ PR Repts, AmpR, CmR, expression of site-specific Flp recombinase under control of heat-inducible promoter, temperature-sensitive replication. | Cherepanov and Wackernagel (1995) |