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. 2013 Dec 15;3(1):29–41. doi: 10.1002/mbo3.145

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© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Xac mutants disrupted for parB are unable to colonize the host citrus. Three Xac parB::pPAU2 mutants were inoculated in leaves of Rangpur lime alongside the wild-type Xac and the diluent PBS 1X. Only Xac wild type was capable of inducing the typical symptoms of citrus canker as eruptive lesions within chlorotic spaces. Infected plants were kept in green house for a period of 60 days in order to score for the appearance of citrus canker symptoms. Here, we show a representative experiment; tests were performed in triplicates.