Figure 2.

Serum represses lasI and lasB expression during early phase (A) but enhances lasI, lasR, and lasB expression during late phase (B) of growth. PAO1 was grown in either LB or LB/S. Samples were collected at early phase (OD₆₀₀ 1.0–1.2) or late phase (OD₆₀₀ 3.0–4.0) and the level of gene expression was determined by qRT-PCR. Values represent the means of three independent experiments ± SEM; ***P < 0.001.