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Serum differentially regulates the expression of the QS-controlled virulence genes (A) lasA, (B) hcnB, (C) plcB, and (D) phzC during both early and late stages of growth. PAO1 was grown in either LB or LB/S, samples were collected at early and late phases, and the level of gene expression was determined by qRT-PCR. Values represent the means of three independent experiments ± SEM; ***P < 0.001, **P < 0.01, *P < 0.05.
