Figure 3.

Promoter activity of sprP. Pseudomonas aeruginosa PAO1 containing plasmid pTZsprP, which harbors a 556 bp DNA fragment upstream of sprP fused to lacZ was inoculated to an initial cell density of OD_580nm = 0.1 and incubated for 24 h at 37°C. Activity of β-galactosidase (bars) was determined as described by Miller (1972) and the corresponding growth of the culture (line) was determined as absorbance of the bacterial culture at 580 nm. Error bars indicate standard deviation.