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. 2014 Mar 1;127(5):1018–1032. doi: 10.1242/jcs.138776

Fig. 4.

Fig. 4.

RAB26 localizes with other lysosomal markers. (A) Confocal image of a HGC-27 cell transfected with EGFP–RAB26 (green) and immunostained for LAMP1 (red) and cathepsin D (blue). The cell border is outlined; N, nucleus. Insets show a higher magnification of a lysosomal vesicle (arrowhead) that contains membranous RAB26 and LAMP1 with interior cathepsin D. (B) Immunofluorescence staining HGC-27 cells transfected with EGFP–RAB26 (green) and co-stained for cathepsin D (red). (C) Epifluorescence microscopy of HGC-27 cells co-transfected with EGFP–RAB26 (green) and RFP-tagged cathepsin D (CTSD–RFP, red). (E) Immunofluorescence staining HGC-27 cells transfected with EGFP–RAB26 (green) and co-stained for RAB7 (red). Scale bars: 20 µm.