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. 2014 Mar 1;127(5):1033–1042. doi: 10.1242/jcs.140251

Fig. 4.

Fig. 4.

PDE4B controls β1AR , but not β2AR or EPR responses in cardiac myocytes. Wild-type and PDE4BKO myocytes expressing Epac2-PM were preincubated with 1 µM ICI-118551 (ICI, β2 antagonist) (A) or 1 µM CGP-20712A (CGP, β1 antagonist) (B) for 5 min, then stimulated with Iso (10 nM) followed by IBMX (100 µM) plus Fsk (50 µM). (C) Wild-type and PDE4BKO myocytes were stimulated with PGE2 (100 nM) followed by IBMX (100 µM) plus Fsk (50 µM). The average ratio R/R0 (R = CFP/FRET) is reported and the number of cells analyzed (n) includes data from at least four experiments. Data were analyzed using a two-way ANOVA. Error bars represent the s.e.m. Fsk, forskolin.