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. 2014 Mar 1;127(5):1092–1103. doi: 10.1242/jcs.142828

Fig. 3.

Fig. 3.

The PY-NLS motif is required for efficient nuclear translocation of Ci and optimal Ci activity in vivo. (A–D′) Wing discs expressing UAS-HA-CiWT (A,A′), UAS-HA-CimNLS-N (B,B′), UAS-HA-CimNLS-C (C,C′) or UAS-HA-CimNLS-N+C (D,D′) with the C765 Gal4 driver were immunostained to show the expression of Ptc (A–D) and Ci (A′–D′). Arrows indicate the posterior compartments. (E–H′) Low (E–H) and high (E′–H′) magnification views of wing discs expressing UAS-HA-CiWT (E,E′), UAS-HA-CimNLS-N (F,F′), UAS-HA-Ci-mNLS-C (G,G′) or UAS-HA-CimNLS-N+C (H,H′) with the MS1096 Gal4 driver were treated with LMB 50 ng/ml for 2 hours prior to immunostaining with an anti-Ci antibody and a nuclear dye (not shown). Arrows in E–H indicate the posterior compartments that are enlarged in panels E′–H′. Arrowheads in E′ and H′ highlight individual cells exhibiting predominant nuclear localization of CiWT and predominant cytoplasmic localization of CimNLS-N+C, respectively.