Fig. 4.

Iqg1 phosphomutants accumulate at bud neck prematurely. (A) Live-cell images of cells expressing C-terminal fusions of eGFP and mCherry at the endogenous loci of IQG1 and SPC42, respectively. Arrowhead, pre-anaphase Iqg1p–eGFP accumulation at the bud neck in the iqg1 phosphomutant. Scale bars: 2 µm. (B) Quantification of green fluorescence signal in the bud neck region over time for eight representative cells from each genotype, normalized and smoothed. (C) Time series of Iqg1–eGFP dynamics at the bud neck in typical wild-type and iqg1 phosphomutant cells, at 2-min intervals. (D) Population average of normalized mean eGFP fluorescence at each time point for the indicated number of cells, pooling data from two experiments (±s.d.). (E) Population average of normalized mean eGFP fluorescence at each time point for all four iqg1 alleles tested.