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Iqg1 associates with Mlc1 and Hof1 in vivo. Sypro-Ruby stain of Iqg1–GFP immunoprecipitates from lysates of nocodazole-arrested cells. Hof1 and Mlc1 were identified by mass spectrometry (supplementary material Table S3). There were no reproducible differences between the IQG1-eGFP and iqg1-14A-eGFP preparations.
