Fig. 7.

Localization of ATP8A2 to the Golgi and endosomes of photoreceptor inner segments and PC12 cells by immunofluorescence microscopy. (A) Photoreceptors in retina cryosections of WT mice were labeled with a polyclonal antibody against ATP8A2 (green) and a monoclonal antibody against the Golgi marker GM130 (red) and counterstained with 4′,6-diamidino-2-phenylindole (blue). The merged image shows partial colocalization of ATP8A2 and GM130 (yellow). OS, outer segments; IS, inner segments; ONL, outer nuclear layer. (B) ATP8A2 (green) expressed in PC12 cells partially colocalizes (yellow) with GM130 and Rab11 (red). Arrows indicate the neurite tips. Scale bars: 10 µm (A), 50 µm (B).