Figure 1. Short exposure to KSHV induces STAT3 activation in human DCs.

DCs were mock treated or exposed to (A) active or (B) UV-inactivated virus (UV-KSHV) for 15 and 30 min and STAT3, NF-kB p65 and AKT phosphorylation were analysed by western blot. Total STAT3, NF-kB p65, AKT are also shown. STAT3, NF-kB p65 and AKT phosphorylation and their fold of induction, based on densitometric analysis of the ratio of phospho/total molecules, are shown. GAPDH was included as protein loading control. 1 × 10⁶ overnight serum-starved DCs/point were used in all experiments. One representative experiment out of three is shown.