Figure 5. STAT3 silencing prevents the KSHV-mediated autophagic block in DCs.
(A) Western blot analysis showing pSTAT3, p62 and Mcl1 expression and (B) LC3I/II in DC treated with control (scramble) or STAT3 specific siRNA exposed to UV-KSHV for 15 min and cultured 24 hours in serum free conditions. (C) LC3I/II was also evaluated in the presence of 3-MA autophagic blocker. GAPDH was included as protein loading control. 1 × 106 overnight serum-starved DCs/point were used in all experiments. Data, representative of three independent experiments, are reported.