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. 2014 Feb 24;7:80. doi: 10.1186/1756-3305-7-80

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Copyright © 2014 Zhao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Binding of native Ts-Pmy to human C9 was identified using immunoprecipitation. The extracts of T. spiralis adult worms were incubated with human C9 and anti-C9 mAb and precipitated with protein G beads. The binding complex was separated by 12% SDS-PAGE and probed with anti-Ts-Pmy mAb 7E2. M, standard protein marker; Lane 1, T. spiralis adult worm extracts only; Lane 2, worm extracts incubated with anti-C9 mAb only as a control; Lane 3, worm extracts incubated with human C9 and anti-C9 mAb.