Table 2.
Enzyme activities in mature green and red fruits ripened on- and off-the-vine. One U is the amount of enzyme which catalyzes the transformation of 1 μmol of substrate per min. Results are presented as means of three independent experiments ± SE.
| Enzyme | Green | Red on-the-vine mU/mg protein |
Red off-the-vine |
|---|---|---|---|
| Aminating GDH | 26.33 ± 5.56 | 25.04 ± 3.61 | 30.50 ± 1.71 |
| Deaminating GDH | 89.20 ± 11.71 | 7.22 ± 1.25 | 12.63 ± 2.55 |
| GDH aminating/deaminating | 0.29 ± 0.02 | 3.49 ± 0.10 | 2.49 ± 0.37 |
| GAD | 86.74 ± 15.27 | 5.11 ± 2.60 | 32.28 ± 7.72* |
| GABA-T | 8.30 ± 3.00 | 47.15 ± 1.25 | 13.19 ± 0.28* |
| SSADH | 9.88 ± 0.65 | 1.50 ± 0.47 | 2.25 ± 0.74 |
* The asterisks indicate statistical significance of means between both ripening conditions.