Table 3.

Pearson’s correlation between extracellular lactate, Glo1 and LDH activity, number of migrated cells (migration) and relative metabolite intensities for the five breast cancer cell lines (sample set 1), and for the breast cancer cell lines and an Glo1 knockdown mutant MCF-7siGlo1 and the corresponding mock transfected control cells of the cell line MCF-7, MCF-7 mock (sample set 2). The dataset was calculated on basis of mean values, n = 6 for the metabolite profiles and Glo1 activity per cell line, n = 5 for cell migration, n = 4 for LDH activity.

	Sample set	extracellular lactate*		Glo1 activity		LDH activity		Cell migration
		r	p	r	p	r	p	r	p
Glycerophosphoglycerol (5TMS)	2	0.77	0.009	0.87	0.001	0.91	9.50×10−5	0.98	5.03×10−7
	1	0.81	0.085	0.88	0.016	0.95	0.002	0.78	0.057
Pantothenic acid D (3TMS)	2	0.87	0.001	0.84	0.001	0.85	0.001	0.86	0.001
	1	0.67	0.282	0.91	0.007	0.85	0.026	0.95	0.002
Alanine, beta- (3TMS)	2	0.91	1.24×10−4	0.63	0.069	0.82	0.003	0.82	0.003
	1	0.99	2.38×10−4	0.63	0.204	0.85	0.023	0.63	0.206
extracellular lactate	2			0.84	0.002	0.80	0.004	0.85	0.001
	1			0.72	0.104	0.89	0.013	0.82	0.037
Glo1 activity	2	0.84	0.002			0.76	0.010	0.86	0.001
	1	0.72	0.197			0.94	0.004	0.86	0.020
LDH activity	2	0.80	0.004	0.76	0.010			0.92	9.25×10−5
	1	0.89	0.029	0.94	0.004			0.81	0.040
Cell migration	2	0.85	0.001	0.86	0.001	0.92	9.25×10−5
	1	0.82	0.078	0.86	0.020	0.81	0.040

*extracellular lactate was not analyzed for MDA-MB-435, therefore n = 4 for this correlation using values from sample set 1.