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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: Dev Dyn. 2008 Mar;237(3):837–846. doi: 10.1002/dvdy.21457

Fig. 6.

Fig. 6

Analysis of kinase activity. NIH3T3 cells transfected with HA-focal adhesion kinase (FAK), HA-ΔFAK, or HA-KIN-32 constructs were plated on fibronectin-coated cell culture plates for 30 min, lysed, and immunoprecipitated with anti-HA antibodies. A: Half of the immunoprecipitate was immunoblotted with anti-HA antibodies. The immunoprecipitate in the green fluorescent protein (GFP) lane is a negative control. B: The other half of the immunoprecipitate was used for in vitro phosphorylation of the tyrosine kinase substrate poly(Glu-Tyr) with γ32P-ATP. Kinase activity relative to HA-FAK is indicated by the shaded bars (± SD).