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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: Ann Anat. 2010 Oct 25;193(1):23–29. doi: 10.1016/j.aanat.2010.09.005

Fig. 2.

Fig. 2

Detection by Western blotting of estrogen receptors alpha and beta (ERα, ERβ), androgen receptor (AR) and insulin receptor (IR) proteins in chondrocyte cell lines C-28/I2 and T/C-28a2 and in human articular cartilage chondrocytes cultured without (A) and with hormones (B). (A) ER alpha (66 or 46 kDa) and beta (57 kDa), AR (72 kDa) and IR (95 kDa) proteins were detected in C-28/I2 and T/C-28a2 chon-drocytes (lanes 2, 3), in human articular cartilage chondrocytes (lane 4) and in placentar tissue used as positive control (lane 1). (B) Positive controls were protein extracts from uterine (U), prostate (P) and liver (L) tissues. Untreated controls were C1 (serum-free medium) and C2 (serum-free medium with solvents, ethanol or PBS/NaOH). ERα was detected at 66 kDa using antibody MAB 461. C28/I2 cells were incubated without (lanes 2, 3) or with 10−11, 10−9, 10−7, 10−6 and 10−4 M 17β-estradiol (lanes 4–8). ERβ was detected at 57 kDa with antibody AB 1410. C28/I2 cells were incubated without (lanes 2, 3, 5, 6) or with 5 μg/ml insulin (lanes 4, 9). AR was detected at 72 kDa with antibody AR N-20. T/C-28a2 cells were incubated without (lanes 2, 3) or with 10−8 M dihydrotestosterone (lane 4). C-28/I2 cells were incubated without (lanes 5, 6) or with 10−8 M dihydrotestosterone (lane 7). IR was detected at 95 kDa with antibody IR GR 36. C-28/I2 cells were incubated without (lane 2) or with 10−11, 10−9, 10−7, 10−6 and 10−4 M 17β-estradiol (lanes 3–7).